Device

Part:BBa_K1698001:Design

Designed by: Leanne O Sullivan   Group: iGEM15_Cork_Ireland   (2015-09-18)


BaseHunter : Human Papiloma Virus (HPV) Detector Part - Can be activated to become detector for 55bp


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 706
    Illegal SapI site found at 922


Design Notes

Target sequence is ideally chosen between two restriction sites to allow digestion of a large genomic sample to yield target fragment of correct size. Also, the size of the target was chosen as it works efficiently with detector.


Source

Target sequence comes from HPV-16 Genome : NC_001526

References